Composite

Part:BBa_K283011

Designed by: Shi Lei, W Ling , Nelson Chu   Group: iGEM09_HKU-HKBU   (2009-10-13)

pTet-rbs-lpp_omp(46-159)_streptavidin_Histag

The fusion protein lpp-ompA(46-159)-streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The backbone of this biobrick is based on BBa_J61003.lpp_ompA(46-159)_streptavidin(WT)_his tag lies between XbaI and SpeI.This part does not pose any biological threat.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 607
    Illegal AgeI site found at 658
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/generator/prc
//regulation/negative
Parameters
None