Composite
Part:BBa_K283011
Designed by: Shi Lei, W Ling , Nelson Chu Group: iGEM09_HKU-HKBU (2009-10-13)
pTet-rbs-lpp_omp(46-159)_streptavidin_Histag
The fusion protein lpp-ompA(46-159)-streptavidin(WT) with a 6-histidine tail is linked after a pTet promoter. The fusion protein coding region does not contain any terminator. The backbone of this biobrick is based on BBa_J61003.lpp_ompA(46-159)_streptavidin(WT)_his tag lies between XbaI and SpeI.This part does not pose any biological threat.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 565
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 607
Illegal AgeI site found at 658 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/generator/prc
//regulation/negative
//regulation/negative
None |